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Image Search Results
Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: Main GUI of the FluoAnalysis toolbox. A multi-tiff file, imported into the GUI with the identified ROIs marked by yellow contours ( top left ). Average projection of the 546 imported frame is visible at the ( top right ), followed by a thresholded binary image and the ROIs in pseudo colors below. ROI detection and ΔF/F 0 calculation parameters are located in the middle column. The calculated ΔF/F 0 traces for all identified ROIs during a control period and application of 1 mM ATP to a hippocampal brain slice expressing GCaMP2 calcium-sensitive fluorescent protein in neurons and astrocytes are visible in the bottom left.
Article Snippet: In conclusion, the
Techniques: Control, Slice Preparation, Expressing
Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: A GUI for classification and spectral analysis of Ca 2+ imaging data of individual cells. High Ca 2+ imaging sampling rate (68 Hz) together with the unique spectral analysis capabilities of the FluoAnalysis toolbox enables the identification of a high-frequency imaging signal that corresponds to the firing rate of a cortical pyramidal cell as measured by patch clamp electrophysiology. All imaged cells (listed on the right) can be individually investigated. Automatic classification of the cells as neurons or astrocytes, based on the ratio of green and red channel intensities in the ROIs, can be manually overridden. Wavelet analysis can be easily applied at different frequency bands.
Article Snippet: In conclusion, the
Techniques: Imaging, Sampling, Patch Clamp
Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: Comparison of features of the FluoAnalysis toolbox with other calcium imaging analysis tools.
Article Snippet: In conclusion, the
Techniques: Comparison, Imaging
Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: Main GUI of the FluoAnalysis toolbox. A multi-tiff file, imported into the GUI with the identified ROIs marked by yellow contours ( top left ). Average projection of the 546 imported frame is visible at the ( top right ), followed by a thresholded binary image and the ROIs in pseudo colors below. ROI detection and ΔF/F 0 calculation parameters are located in the middle column. The calculated ΔF/F 0 traces for all identified ROIs during a control period and application of 1 mM ATP to a hippocampal brain slice expressing GCaMP2 calcium-sensitive fluorescent protein in neurons and astrocytes are visible in the bottom left.
Article Snippet: The
Techniques: Control, Slice Preparation, Expressing
Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: A GUI for classification and spectral analysis of Ca 2+ imaging data of individual cells. High Ca 2+ imaging sampling rate (68 Hz) together with the unique spectral analysis capabilities of the FluoAnalysis toolbox enables the identification of a high-frequency imaging signal that corresponds to the firing rate of a cortical pyramidal cell as measured by patch clamp electrophysiology. All imaged cells (listed on the right) can be individually investigated. Automatic classification of the cells as neurons or astrocytes, based on the ratio of green and red channel intensities in the ROIs, can be manually overridden. Wavelet analysis can be easily applied at different frequency bands.
Article Snippet: The
Techniques: Imaging, Sampling, Patch Clamp
Journal: Brain Sciences
Article Title: FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks
doi: 10.3390/brainsci14080830
Figure Lengend Snippet: Comparison of features of the FluoAnalysis toolbox with other calcium imaging analysis tools.
Article Snippet: The
Techniques: Comparison, Imaging